bandshift assay

bandshift assay
(= gel shift assay)
An assay for proteins, such as transcription factors, that bind specific DNA sequences. A labelled oligonucleotide corresponding to the recognition sequence is incubated with an appropriate nuclear protein extract, and run on a non-denaturing acrylamide gel. Oligonucleotides that have been bound by proteins are retarded relative to those that are unbound.

Dictionary of molecular biology. 2004.

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  • bandshift assay — noun A type of assay using gel electrophoresis, in which the mobility of a DNA or RNA probe alone is compared to its mobility in combination with a particular protein, so as to measure the degree and nature of binding between the protein and DNA… …   Wiktionary

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